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elisa hfsh kit  (VEDALAB Inc)

 
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    VEDALAB Inc elisa hfsh kit
    Elisa Hfsh Kit, supplied by VEDALAB Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa hfsh kit/product/VEDALAB Inc
    Average 90 stars, based on 1 article reviews
    elisa hfsh kit - by Bioz Stars, 2026-03
    90/100 stars

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    <t>FSH</t> induces neuroinflammation in mice. Four weeks after the FSH treatment, the brain and serum of the mice were harvested for western blot (a,b), <t>Elisa</t> assay (c), and immunofluorescence staining (d,e). (a) Representative immunoblots showing the protein expression of IL-1β and IL-6 in the hippocampus. (b) Quantification of the immunoreactivity of IL-1β and IL-6 protein expression, data are shown as represent mean ± SEM, n = 4 mice per group. (c) ELISA quantification shows the levels of proinflammation factors IL-1β, IL-6, and TNFα in the serum, data are shown as mean ± SEM, n = 6 mice per group. (d) Immunofluorescent co-staining of Iba1 (gray) and GFAP (red) on the hippocampal sections (scale bar: 150 μm). (e) Quantification of Iba1 positive and GFAP positive cells, data represent mean ± SEM ( n = 4 mice per group, 10 sections each mouse). Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple comparisons test. *p < 0.05, * *p < 0.01; ns, not significant.
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    Image Search Results


    FSH induces neuroinflammation in mice. Four weeks after the FSH treatment, the brain and serum of the mice were harvested for western blot (a,b), Elisa assay (c), and immunofluorescence staining (d,e). (a) Representative immunoblots showing the protein expression of IL-1β and IL-6 in the hippocampus. (b) Quantification of the immunoreactivity of IL-1β and IL-6 protein expression, data are shown as represent mean ± SEM, n = 4 mice per group. (c) ELISA quantification shows the levels of proinflammation factors IL-1β, IL-6, and TNFα in the serum, data are shown as mean ± SEM, n = 6 mice per group. (d) Immunofluorescent co-staining of Iba1 (gray) and GFAP (red) on the hippocampal sections (scale bar: 150 μm). (e) Quantification of Iba1 positive and GFAP positive cells, data represent mean ± SEM ( n = 4 mice per group, 10 sections each mouse). Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple comparisons test. *p < 0.05, * *p < 0.01; ns, not significant.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Follicle-stimulating hormone induces depression-like phenotype by affecting synaptic function

    doi: 10.3389/fnmol.2024.1459858

    Figure Lengend Snippet: FSH induces neuroinflammation in mice. Four weeks after the FSH treatment, the brain and serum of the mice were harvested for western blot (a,b), Elisa assay (c), and immunofluorescence staining (d,e). (a) Representative immunoblots showing the protein expression of IL-1β and IL-6 in the hippocampus. (b) Quantification of the immunoreactivity of IL-1β and IL-6 protein expression, data are shown as represent mean ± SEM, n = 4 mice per group. (c) ELISA quantification shows the levels of proinflammation factors IL-1β, IL-6, and TNFα in the serum, data are shown as mean ± SEM, n = 6 mice per group. (d) Immunofluorescent co-staining of Iba1 (gray) and GFAP (red) on the hippocampal sections (scale bar: 150 μm). (e) Quantification of Iba1 positive and GFAP positive cells, data represent mean ± SEM ( n = 4 mice per group, 10 sections each mouse). Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple comparisons test. *p < 0.05, * *p < 0.01; ns, not significant.

    Article Snippet: Recombinant human FSH protein (hor-249) was from Prospecbio; antibody to IL-6 (YT5348) was from Immuneway; antibodies to IL-1 β (16806-1-AP), GluR1 (67642-1-Ig), FSHR (22665-1-AP), GAD67 (10408-1-AP), VGluT1 (21829-1-AP), and synaptophysin (17785-1-AP) were from Proteintech; antibodies to GluR2 (A11316), synapsin (WX620330), PSD95 (A7889), ERK1/2 (A4782), and phospho-ERK1/2 (AP0472) were from Abclonal; antibody to GFAP (AB-2532994) was from Invitrogen; antibody to Iba1 (019-19741) was from Wako; antibody to β-actin (GB11001-100) was from Servicebio; ERK1/2 inhibitor (HY-112287) was from MedChemExpress; IL-1β mouse ELISA kit (MU30369), IL-6 mouse ELISA kit (MU30044), and TNF- α mouse ELISA kit (MU30030) were from Bioswamp; human FSH ELISA kit (CSB-E06867h) and luteinizing hormone (LH) ELISA kit (CSB-E12770m) were from Cusabio; 17β-estradiol ELISA kit (ab108667) was from Abcam; 4′,6-diamidino-2-phenylindole (DAPI, D9542) was from Sigma-Aldrich; AAV9 sh-FSHR (BC-2403) and AAV9 sh-Control (BC-0183) adenovirus were customized, synthesized, and purified by Shenzhen Brain Case Biotechnology Inc.

    Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Expressing

    FSHR knockdown alleviates FSH-induced neuroinflammation in mice. (a,b) Representative immunoblots images (a) and quantification (b) of IL-1β and IL-6 protein expression showing a reduction in the hippocampus of the sh-FSHR + FSH group compared to sh-Control + FSH group, data are presented as mean ± SEM, n = 4 mice per group. (c) ELISA quantification of pro-inflammation factors IL-1β, IL-6, and TNFα in the serum, data are presented as mean ± SEM, n = 6 mice per group. (d,e) Immunofluorescent co-staining (d) and quantification (e) of Iba1 (gray) and GFAP (red) on the hippocampal sections (scale bar: 150 μm), data are presented as mean ± SEM ( n = 4 mice per group, 10 sections each mouse). Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple comparisons test, *p < 0.05, * *p < 0.01.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Follicle-stimulating hormone induces depression-like phenotype by affecting synaptic function

    doi: 10.3389/fnmol.2024.1459858

    Figure Lengend Snippet: FSHR knockdown alleviates FSH-induced neuroinflammation in mice. (a,b) Representative immunoblots images (a) and quantification (b) of IL-1β and IL-6 protein expression showing a reduction in the hippocampus of the sh-FSHR + FSH group compared to sh-Control + FSH group, data are presented as mean ± SEM, n = 4 mice per group. (c) ELISA quantification of pro-inflammation factors IL-1β, IL-6, and TNFα in the serum, data are presented as mean ± SEM, n = 6 mice per group. (d,e) Immunofluorescent co-staining (d) and quantification (e) of Iba1 (gray) and GFAP (red) on the hippocampal sections (scale bar: 150 μm), data are presented as mean ± SEM ( n = 4 mice per group, 10 sections each mouse). Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple comparisons test, *p < 0.05, * *p < 0.01.

    Article Snippet: Recombinant human FSH protein (hor-249) was from Prospecbio; antibody to IL-6 (YT5348) was from Immuneway; antibodies to IL-1 β (16806-1-AP), GluR1 (67642-1-Ig), FSHR (22665-1-AP), GAD67 (10408-1-AP), VGluT1 (21829-1-AP), and synaptophysin (17785-1-AP) were from Proteintech; antibodies to GluR2 (A11316), synapsin (WX620330), PSD95 (A7889), ERK1/2 (A4782), and phospho-ERK1/2 (AP0472) were from Abclonal; antibody to GFAP (AB-2532994) was from Invitrogen; antibody to Iba1 (019-19741) was from Wako; antibody to β-actin (GB11001-100) was from Servicebio; ERK1/2 inhibitor (HY-112287) was from MedChemExpress; IL-1β mouse ELISA kit (MU30369), IL-6 mouse ELISA kit (MU30044), and TNF- α mouse ELISA kit (MU30030) were from Bioswamp; human FSH ELISA kit (CSB-E06867h) and luteinizing hormone (LH) ELISA kit (CSB-E12770m) were from Cusabio; 17β-estradiol ELISA kit (ab108667) was from Abcam; 4′,6-diamidino-2-phenylindole (DAPI, D9542) was from Sigma-Aldrich; AAV9 sh-FSHR (BC-2403) and AAV9 sh-Control (BC-0183) adenovirus were customized, synthesized, and purified by Shenzhen Brain Case Biotechnology Inc.

    Techniques: Knockdown, Western Blot, Expressing, Control, Enzyme-linked Immunosorbent Assay, Staining

    Chemotherapy-induced premature ovarian insufficiency (POI) mice model. A Vaginal exfoliative cell smear and ovary section of normal mice (a–d, × 100; i, × 40; j, × 200) and POI mice (e–h, × 100; k, × 40, l, × 200). B Comparison of the follicles and the proportion of atretic follicles between CTX/BUS+ group and CTX/BUS− groups (* P < 0.05). C E2 and FSH levels determined by ELISA 14 days after CTX/BUS administration (* P < 0.05). E2 oestradiol, FSH follicle-stimulating hormone, CTX/BUS+ CTX/BUS administration, CTX/BUS− CTX/BUS non-administration

    Journal: Stem Cell Research & Therapy

    Article Title: Therapeutic effects of human umbilical cord mesenchymal stem cell-derived microvesicles on premature ovarian insufficiency in mice

    doi: 10.1186/s13287-019-1327-5

    Figure Lengend Snippet: Chemotherapy-induced premature ovarian insufficiency (POI) mice model. A Vaginal exfoliative cell smear and ovary section of normal mice (a–d, × 100; i, × 40; j, × 200) and POI mice (e–h, × 100; k, × 40, l, × 200). B Comparison of the follicles and the proportion of atretic follicles between CTX/BUS+ group and CTX/BUS− groups (* P < 0.05). C E2 and FSH levels determined by ELISA 14 days after CTX/BUS administration (* P < 0.05). E2 oestradiol, FSH follicle-stimulating hormone, CTX/BUS+ CTX/BUS administration, CTX/BUS− CTX/BUS non-administration

    Article Snippet: The levels of estradiol (E2) and follicle-stimulating hormone (FSH) were measured with ELISA Kit (Cusabio, China).

    Techniques: Comparison, Enzyme-linked Immunosorbent Assay

    Changes of hormone levels after HUCMSC-MVs transplantation. Serum levels of estradiol (E2) and follicle stimulating hormone (FSH) in three groups (* P < 0.05 vs control group, # P < 0.05 vs POI-MVs group

    Journal: Stem Cell Research & Therapy

    Article Title: Therapeutic effects of human umbilical cord mesenchymal stem cell-derived microvesicles on premature ovarian insufficiency in mice

    doi: 10.1186/s13287-019-1327-5

    Figure Lengend Snippet: Changes of hormone levels after HUCMSC-MVs transplantation. Serum levels of estradiol (E2) and follicle stimulating hormone (FSH) in three groups (* P < 0.05 vs control group, # P < 0.05 vs POI-MVs group

    Article Snippet: The levels of estradiol (E2) and follicle-stimulating hormone (FSH) were measured with ELISA Kit (Cusabio, China).

    Techniques: Transplantation Assay, Control

    Regression analysis for conductometric technique and ELISA, correlation coefficient r2 is 0.703

    Journal: The Malaysian Journal of Medical Sciences : MJMS

    Article Title: The Use of Electronic Sensor in Hormone Analysis

    doi:

    Figure Lengend Snippet: Regression analysis for conductometric technique and ELISA, correlation coefficient r2 is 0.703

    Article Snippet: Chemicals used include bovine serum albumine (BSA), Tween 20, Urease type VII, urea, human Folicle Stimulating Hormone (hFSH) from Sigma (USA), hFSH ELISA kit from Veda Lab (USA) and polyclonal antibody to FSH from the Endocrine Unit, Universiti Kebangsaan Malaysia (UKM).

    Techniques: Enzyme-linked Immunosorbent Assay

    Regression analysis of patient samples for conductometric technique and ELISA, correlation coefficient r2 is 0.894

    Journal: The Malaysian Journal of Medical Sciences : MJMS

    Article Title: The Use of Electronic Sensor in Hormone Analysis

    doi:

    Figure Lengend Snippet: Regression analysis of patient samples for conductometric technique and ELISA, correlation coefficient r2 is 0.894

    Article Snippet: Chemicals used include bovine serum albumine (BSA), Tween 20, Urease type VII, urea, human Folicle Stimulating Hormone (hFSH) from Sigma (USA), hFSH ELISA kit from Veda Lab (USA) and polyclonal antibody to FSH from the Endocrine Unit, Universiti Kebangsaan Malaysia (UKM).

    Techniques: Enzyme-linked Immunosorbent Assay